S. Karthikeyan ¹, R. Prasad ¹, T. S. Mahendran ¹, K. Rajagopal ¹, V. Ravendran ²

Affiliations
1 Department of Biotechnology, Vel’ s University, Pallavaram, Chennai 600 117, IN
2 Department of Botany, Madras Christian College, Chennai 600 059, IN

Abstract

This study reports a simple micropropagation protocol and thereby rapid multiplication of the useful medicinal plant- Scoparia dulcis L. Single node explants were inoculated on basal MS medium containing 3% (w/v) sucrose, supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), kinetin (KN), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and Naphthalene acetic acid (NAA) for direct plant regeneration. Maximum numbers of shoot (~22) were observed on the medium containing 0.5 mg/l BAP and 0.25 mg/l IAA after four weeks of culture. Regenerated shoots were separated and ischolar_mained on half strength MS medium supplemented with 0.5 mg/l of IBA alone for three weeks. Simultaneous regeneration of shoots and ischolar_mains and in vitro flowering were achieved from the nodal explants on MS medium supplemented with 0.5 mg/l KN and 2.0 mg/l IAA. Well-developed complete plantlets were transferred on to specially made plastic cup containing soil rite. Acclimatized plantlets were successfully grown in garden soil.

Keywords

Scoparia dulcis, Nodal Explants, Micropropagation, Plant Tissue Culture

Full Text